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rabbit anti γh2ax polyclonal antibody cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti γh2ax polyclonal antibody cell signaling technology
    Rabbit Anti γh2ax Polyclonal Antibody Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+pab+cell+signaling+technology/pmc12767804-416-14-18
    Average 86 stars, based on 1 article reviews
    rabbit anti γh2ax polyclonal antibody cell signaling technology - by Bioz Stars, 2026-10
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    Western Blot:

    Article Title: Mucin 4 Confers Gemcitabine Resistance and an Unfavorable Prognosis in Patients with Cholangiocarcinoma via AKT Activation
    Article Snippet: .. Origin Incorporation α-tubulin WB (1:5000) T6793 mouse mAb Sigma‒Aldrich (St. Louis, MO) Actin WB (1:5000) MAB1501R mouse mAb EMD Millipore (Billerica, MA) AKTpS473 WB (1:1000) #4060 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) pan-AKT WB (1:2000) #4685 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) AKT1 WB (1:2000) #2938 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) AKT2 WB (1:2000) #3063 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) BAX pS184 WB (1:1000) PA5-39778 rabbit pAb Thermo Fisher Scientific Inc. (Waltham, MA) BAX WB (1:1000) #2772 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) CK-19 WB (1:2000) ab133496 rabbit pAb Abcam Plc. (Cambridge, UK) ERK1/2 pT202/Y204 WB (1:2000) #4370 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ERK1/2 WB (1:2000) #4695 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) EGFR pY1068 WB (1:1000) #3777 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) EGFR WB (1:2000) #4267 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 pY877 WB (1:1000) #2241 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 pY1221/1222 WB (1:1000) #2243 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 WB (1:2000) #2165 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER3 pY1289 WB (1:1000) #4791 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER3 WB (1:2000) #12708 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) hENT1 WB (1:2000) 11337-1-AP rabbit pAb Proteintech Group, Inc. (Rosemont, USA) Hep Par1 WB (1:1000) MAB7927 mouse mAb Abnova Corporation (Taipei, Taiwan) HNF4α WB (1:1000) ab41898 mouse mAb Abcam Plc. (Cambridge, UK) MUC4 IHC (1:250) 35-4900 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) MUC4 WB (1:1000) #81692 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) PARP1 WB (1:1000) #9532 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Abbreviations: WB, western blot; IHC, immunohistochemistry; mAb: monoclonal antibody; pAb, polyclonal antibody Supplementary Table 4. ..

    Article Title: PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions
    Article Snippet: .. Origin Company α-Tubulin WB (1:5000) T6793 mouse mAb Sigma‒Aldrich (St. Louis, MO) beta-Catenin WB (1:2000) #8480 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Claudin-1 WB (1:1000) #13255 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) MISP IHC (1:1500) ab254919 rabbit pAb Abcam plc. (Cambridge, UK) MISP WB (1:2000); IF (1:50) 26338-1-AP rabbit pAb Proteintech Group, Inc. (Rosemont, USA) MLC pS19 WB (1:1000) #3671 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) MLC pS19 IHC (1:400) 600-401-416 rabbit pAb Thermo Fisher Scientific Inc. (Waltham, MA) MLC WB (1:1000) #8505 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (24E10) WB (1:2000); IF (1:100) #3195 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (ECCD-2) IF (1:200) #13-1900 rat mAb Thermo Fisher Scientific Inc. (Waltham, MA) FAK pY397 WB (1:1000) #3283 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) FAK WB (1:2000) #3285 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) N-Cadherin WB (1:2000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Histone H3 pS10 IF (1:800) #9701 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) PLK1 WB (1:2000); IHC (1:100); IF (1:50) #37-7000 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Paxillin pY118 WB (1:1000) #2541 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) Paxillin WB (1:2000); IF (1:200) 610051 mouse mAb BD Transduction LaboratoriesTM (Franklin Lakes, NJ) Snail WB (1:1000) #3879 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Slug WB (1:1000) #9585 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Vimentin WB (1:1000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZEB1 WB (1:1000) #3396 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 WB (1:1000) #8139 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 IF (1:100) #33-9100 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Abbreviations: WB, wWestern blot; IF, immunofluorescence; IHC, immunohistochemistry; mAb: monoclonal antibody; pAb, polyclonal antibody ..

    Article Title: STAT3-coordinated migration facilitates the dissemination of diffuse large B-cell lymphomas
    Article Snippet: Signaling pathway Candidate Inhibitor References Rac1 NSC23766 1, 2 Cdc42 ML141 2, 3 ROCK H1152 2, 4 Wnt/PCP signaling pathway Wnt signaling Wnt-C59 5, 6 TGFβ signaling pathway TGFβ LY364947 7, 8 PI3K/Akt signaling pathway PI3K LY294002 9-11 B cell receptor signaling pathway PI3K/AKT/mTOR pathway mTOR rapamycin 12, 13 SYK-mediated signaling SYK SYK inhibitor 14-17 B cell receptor signaling pathway Integrin signaling pathway Src SU 6656 18-21 Growth factor-signaling pathways B cell receptor signaling pathway PKC-mediated signaling PKC Gö 6983 22, 23 Integrin signaling pathway FAK PF-573228 19, 24 B cell receptor signaling pathway BTK Ibrutinib 25, 26 JAK-STAT3 signaling pathway JAK JAK inhibitor I 27, 28 JAK-STAT3 signaling pathway STAT3 S31-201 29, 30 MyD88 signaling pathway IRAK-4 IRAK-1-4 Inhibitor I 31, 32 MyD88 signaling pathway IRF-4 Lenalidomide 33, 34 Apoptosis signaling pathway BCL-6 79-6 35, 36 Apoptosis signaling pathway BCL-2 ABT-199 37, 38 BET proteins JQ1 39, 40 Supplementary Table 4 Information of inhibitors used in this study Inhibitor Target Working concentration Incorporation 79-6 BCL-6 50 μM EMD Millipore (Billerica, MA) Fascaplysin cyclin D1 1 μM EMD Millipore (Billerica, MA) Blebbistatin myosin II 10 μM Sigma-Aldrich (St. Louis, MO) ABT-199 BCL-2 1 μM ApexBio Technology (Houston, TX) Gö 6983 PKC 1 μM ApexBio Technology (Houston, TX) H1152 ROCK 2 μM EMD Millipore (Billerica, MA) Ibrutinib BTK 2 μM ApexBio Technology (Houston, TX) IRAK-1-4 Inhibitor I IRAK-4 5 μM ApexBio Technology (Houston, TX) JAK inhibitor I pan-JAK 2 μM EMD Millipore (Billerica, MA) 5 μM in Supplementary Fig 3c,d JQ1 BET proteins 1 μM ApexBio Technology (Houston, TX) Lenalidomide IRF-4 1 μM ApexBio Technology (Houston, TX) LY294002 PI3K 15 μM Selleck Chemicals. ( Houston, TX) LY364947 TFGβ 10 μM Sigma-Aldrich (St. Louis, MO) ML141 cdc42 10 μM BioVision (Milpitas, CA) ML-7 MLCK 20 μM Sigma-Aldrich (St. Louis, MO) Nocodazole (Noc) microtubule 3.3 μM Sigma-Aldrich (St. Louis, MO) NSC23766 Rac1 20 μM Sigma-Aldrich (St. Louis, MO) Paclitaxel (taxol) microtubule 2 μM Sigma-Aldrich (St. Louis, MO) PF-573228 FAK 10 μM ApexBio Technology (Houston, TX) proteinase inhibitor (PI) proteinase 1:800 Sigma-Aldrich (St. Louis, MO) Rapamycin mTOR 100 nM EMD Millipore (Billerica, MA) Ruxolitinib JAK 1 μM Novartis International AG (Basel, Switzerland). .. S31-201 STAT3 100μM EMD Millipore (Billerica, MA) 200 μM in Supplementary Fig 3c, d and 6d SU 6656 Src 10 μM EMD Millipore (Billerica, MA) SYK inhibitor SYK 10 μM EMD Millipore (Billerica, MA) Wnt-C59 Wnt signalling 20 μM ApexBio Technology (Houston, TX) Supplementary Table 5 Information of antibodies used in this study Protein Application (Dilution) Antibody Origin Incorporation Acetylated tubulin WB (1:2000) T6199 mouse mAb Sigma-Aldrich (St. Louis, MO) ARHGEF2 (GEF2) WB (1:2000) #4076 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Caspase 3 WB (1:1000) #9662S rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) CD19 CTC collection (1:50) #555413 mouse mAb BD Transduction LaboratoriesTM (Franklin Lakes, NJ) CD20 IHC (1:200) M0775 mouse mAb Dako (Glostrup Denmark) Cdc42 WB (1:1000) A13981 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Cyclin D1 WB (1:1000) #2978 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) FLAG WB (1:1000), IP F1804 mouse mAb Sigma-Aldrich (St. Louis, MO) HA WB (1:1000), IP 901503 mouse mAb BioLegend, Inc. (San Diego, CA) IL-10 WB (1:1000), neutralising ab34843 rabbit pAb Abcam Plc. (Cambridge, UK) IL-12 WB (1:1000), neutralising MAB1570 mouse mAb R&D Systems, Inc. (Minneapolis, MN) IL-6 WB (1:1000), neutralising ab11449 mouse mAb Abcam Plc. (Cambridge, UK) MLC WB (1:2000) #3672 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) MLC pS19 WB (1:1000), IHC (1:50) #3675 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) Rac1 WB (1:1000) PA1-091X mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Rb WB (1:1000) #9309 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) Rb pS780 WB (1:1000) #8180S rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) RhoA WB (1:1000) sc-418 mouse mAb Santa Cruz Biotechnology Inc. (Dallas, TX) RhoGDI-γ WB (1:1000) ab157493 rabbit pAb Abcam Plc. (Cambridge, UK) RhoH WB (1:1000), IHC (1:200) ab118507 rabbit pAb Abcam Plc. (Cambridge, UK) ROCK1 WB (1:1000) #4035 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) STAT3 WB (1:5000), ChIP #9139 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) STAT3 pY705 WB (1:1000), IHC (1:200) #9145 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) α-tubulin WB (1:5000) T6793 mouse mAb Sigma-Aldrich (St. Louis, MO) Abbreviations: ChIP, chromatin immunoprecipitation;; IF, immunofluorescence; IHC, immunohistochemistry; IP, immunoprecipitation; mAb: monoclonal antibody; pAb, polyclonal antibody; WB, western blot Supplementary Table 6 Information of shRNA sequences Gene name shRNA target seguence Scr TTTATGCAATAAGACCTATTC ..

    Immunohistochemistry:

    Article Title: Mucin 4 Confers Gemcitabine Resistance and an Unfavorable Prognosis in Patients with Cholangiocarcinoma via AKT Activation
    Article Snippet: .. Origin Incorporation α-tubulin WB (1:5000) T6793 mouse mAb Sigma‒Aldrich (St. Louis, MO) Actin WB (1:5000) MAB1501R mouse mAb EMD Millipore (Billerica, MA) AKTpS473 WB (1:1000) #4060 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) pan-AKT WB (1:2000) #4685 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) AKT1 WB (1:2000) #2938 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) AKT2 WB (1:2000) #3063 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) BAX pS184 WB (1:1000) PA5-39778 rabbit pAb Thermo Fisher Scientific Inc. (Waltham, MA) BAX WB (1:1000) #2772 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) CK-19 WB (1:2000) ab133496 rabbit pAb Abcam Plc. (Cambridge, UK) ERK1/2 pT202/Y204 WB (1:2000) #4370 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ERK1/2 WB (1:2000) #4695 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) EGFR pY1068 WB (1:1000) #3777 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) EGFR WB (1:2000) #4267 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 pY877 WB (1:1000) #2241 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 pY1221/1222 WB (1:1000) #2243 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER2 WB (1:2000) #2165 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER3 pY1289 WB (1:1000) #4791 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) HER3 WB (1:2000) #12708 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) hENT1 WB (1:2000) 11337-1-AP rabbit pAb Proteintech Group, Inc. (Rosemont, USA) Hep Par1 WB (1:1000) MAB7927 mouse mAb Abnova Corporation (Taipei, Taiwan) HNF4α WB (1:1000) ab41898 mouse mAb Abcam Plc. (Cambridge, UK) MUC4 IHC (1:250) 35-4900 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) MUC4 WB (1:1000) #81692 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) PARP1 WB (1:1000) #9532 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Abbreviations: WB, western blot; IHC, immunohistochemistry; mAb: monoclonal antibody; pAb, polyclonal antibody Supplementary Table 4. ..

    Article Title: PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions
    Article Snippet: .. Origin Company α-Tubulin WB (1:5000) T6793 mouse mAb Sigma‒Aldrich (St. Louis, MO) beta-Catenin WB (1:2000) #8480 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Claudin-1 WB (1:1000) #13255 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) MISP IHC (1:1500) ab254919 rabbit pAb Abcam plc. (Cambridge, UK) MISP WB (1:2000); IF (1:50) 26338-1-AP rabbit pAb Proteintech Group, Inc. (Rosemont, USA) MLC pS19 WB (1:1000) #3671 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) MLC pS19 IHC (1:400) 600-401-416 rabbit pAb Thermo Fisher Scientific Inc. (Waltham, MA) MLC WB (1:1000) #8505 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (24E10) WB (1:2000); IF (1:100) #3195 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (ECCD-2) IF (1:200) #13-1900 rat mAb Thermo Fisher Scientific Inc. (Waltham, MA) FAK pY397 WB (1:1000) #3283 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) FAK WB (1:2000) #3285 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) N-Cadherin WB (1:2000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Histone H3 pS10 IF (1:800) #9701 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) PLK1 WB (1:2000); IHC (1:100); IF (1:50) #37-7000 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Paxillin pY118 WB (1:1000) #2541 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) Paxillin WB (1:2000); IF (1:200) 610051 mouse mAb BD Transduction LaboratoriesTM (Franklin Lakes, NJ) Snail WB (1:1000) #3879 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Slug WB (1:1000) #9585 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Vimentin WB (1:1000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZEB1 WB (1:1000) #3396 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 WB (1:1000) #8139 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 IF (1:100) #33-9100 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Abbreviations: WB, wWestern blot; IF, immunofluorescence; IHC, immunohistochemistry; mAb: monoclonal antibody; pAb, polyclonal antibody ..

    Article Title: STAT3-coordinated migration facilitates the dissemination of diffuse large B-cell lymphomas
    Article Snippet: Signaling pathway Candidate Inhibitor References Rac1 NSC23766 1, 2 Cdc42 ML141 2, 3 ROCK H1152 2, 4 Wnt/PCP signaling pathway Wnt signaling Wnt-C59 5, 6 TGFβ signaling pathway TGFβ LY364947 7, 8 PI3K/Akt signaling pathway PI3K LY294002 9-11 B cell receptor signaling pathway PI3K/AKT/mTOR pathway mTOR rapamycin 12, 13 SYK-mediated signaling SYK SYK inhibitor 14-17 B cell receptor signaling pathway Integrin signaling pathway Src SU 6656 18-21 Growth factor-signaling pathways B cell receptor signaling pathway PKC-mediated signaling PKC Gö 6983 22, 23 Integrin signaling pathway FAK PF-573228 19, 24 B cell receptor signaling pathway BTK Ibrutinib 25, 26 JAK-STAT3 signaling pathway JAK JAK inhibitor I 27, 28 JAK-STAT3 signaling pathway STAT3 S31-201 29, 30 MyD88 signaling pathway IRAK-4 IRAK-1-4 Inhibitor I 31, 32 MyD88 signaling pathway IRF-4 Lenalidomide 33, 34 Apoptosis signaling pathway BCL-6 79-6 35, 36 Apoptosis signaling pathway BCL-2 ABT-199 37, 38 BET proteins JQ1 39, 40 Supplementary Table 4 Information of inhibitors used in this study Inhibitor Target Working concentration Incorporation 79-6 BCL-6 50 μM EMD Millipore (Billerica, MA) Fascaplysin cyclin D1 1 μM EMD Millipore (Billerica, MA) Blebbistatin myosin II 10 μM Sigma-Aldrich (St. Louis, MO) ABT-199 BCL-2 1 μM ApexBio Technology (Houston, TX) Gö 6983 PKC 1 μM ApexBio Technology (Houston, TX) H1152 ROCK 2 μM EMD Millipore (Billerica, MA) Ibrutinib BTK 2 μM ApexBio Technology (Houston, TX) IRAK-1-4 Inhibitor I IRAK-4 5 μM ApexBio Technology (Houston, TX) JAK inhibitor I pan-JAK 2 μM EMD Millipore (Billerica, MA) 5 μM in Supplementary Fig 3c,d JQ1 BET proteins 1 μM ApexBio Technology (Houston, TX) Lenalidomide IRF-4 1 μM ApexBio Technology (Houston, TX) LY294002 PI3K 15 μM Selleck Chemicals. ( Houston, TX) LY364947 TFGβ 10 μM Sigma-Aldrich (St. Louis, MO) ML141 cdc42 10 μM BioVision (Milpitas, CA) ML-7 MLCK 20 μM Sigma-Aldrich (St. Louis, MO) Nocodazole (Noc) microtubule 3.3 μM Sigma-Aldrich (St. Louis, MO) NSC23766 Rac1 20 μM Sigma-Aldrich (St. Louis, MO) Paclitaxel (taxol) microtubule 2 μM Sigma-Aldrich (St. Louis, MO) PF-573228 FAK 10 μM ApexBio Technology (Houston, TX) proteinase inhibitor (PI) proteinase 1:800 Sigma-Aldrich (St. Louis, MO) Rapamycin mTOR 100 nM EMD Millipore (Billerica, MA) Ruxolitinib JAK 1 μM Novartis International AG (Basel, Switzerland). .. S31-201 STAT3 100μM EMD Millipore (Billerica, MA) 200 μM in Supplementary Fig 3c, d and 6d SU 6656 Src 10 μM EMD Millipore (Billerica, MA) SYK inhibitor SYK 10 μM EMD Millipore (Billerica, MA) Wnt-C59 Wnt signalling 20 μM ApexBio Technology (Houston, TX) Supplementary Table 5 Information of antibodies used in this study Protein Application (Dilution) Antibody Origin Incorporation Acetylated tubulin WB (1:2000) T6199 mouse mAb Sigma-Aldrich (St. Louis, MO) ARHGEF2 (GEF2) WB (1:2000) #4076 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Caspase 3 WB (1:1000) #9662S rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) CD19 CTC collection (1:50) #555413 mouse mAb BD Transduction LaboratoriesTM (Franklin Lakes, NJ) CD20 IHC (1:200) M0775 mouse mAb Dako (Glostrup Denmark) Cdc42 WB (1:1000) A13981 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Cyclin D1 WB (1:1000) #2978 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) FLAG WB (1:1000), IP F1804 mouse mAb Sigma-Aldrich (St. Louis, MO) HA WB (1:1000), IP 901503 mouse mAb BioLegend, Inc. (San Diego, CA) IL-10 WB (1:1000), neutralising ab34843 rabbit pAb Abcam Plc. (Cambridge, UK) IL-12 WB (1:1000), neutralising MAB1570 mouse mAb R&D Systems, Inc. (Minneapolis, MN) IL-6 WB (1:1000), neutralising ab11449 mouse mAb Abcam Plc. (Cambridge, UK) MLC WB (1:2000) #3672 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) MLC pS19 WB (1:1000), IHC (1:50) #3675 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) Rac1 WB (1:1000) PA1-091X mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Rb WB (1:1000) #9309 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) Rb pS780 WB (1:1000) #8180S rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) RhoA WB (1:1000) sc-418 mouse mAb Santa Cruz Biotechnology Inc. (Dallas, TX) RhoGDI-γ WB (1:1000) ab157493 rabbit pAb Abcam Plc. (Cambridge, UK) RhoH WB (1:1000), IHC (1:200) ab118507 rabbit pAb Abcam Plc. (Cambridge, UK) ROCK1 WB (1:1000) #4035 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) STAT3 WB (1:5000), ChIP #9139 mouse mAb Cell Signaling Technology, Inc. (Danvers, MA) STAT3 pY705 WB (1:1000), IHC (1:200) #9145 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) α-tubulin WB (1:5000) T6793 mouse mAb Sigma-Aldrich (St. Louis, MO) Abbreviations: ChIP, chromatin immunoprecipitation;; IF, immunofluorescence; IHC, immunohistochemistry; IP, immunoprecipitation; mAb: monoclonal antibody; pAb, polyclonal antibody; WB, western blot Supplementary Table 6 Information of shRNA sequences Gene name shRNA target seguence Scr TTTATGCAATAAGACCTATTC ..

    Immunofluorescence:

    Article Title: PLK1 and its substrate MISP facilitate intrahepatic cholangiocarcinoma progression by promoting lymphatic invasion and impairing E-cadherin adherens junctions
    Article Snippet: .. Origin Company α-Tubulin WB (1:5000) T6793 mouse mAb Sigma‒Aldrich (St. Louis, MO) beta-Catenin WB (1:2000) #8480 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Claudin-1 WB (1:1000) #13255 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) MISP IHC (1:1500) ab254919 rabbit pAb Abcam plc. (Cambridge, UK) MISP WB (1:2000); IF (1:50) 26338-1-AP rabbit pAb Proteintech Group, Inc. (Rosemont, USA) MLC pS19 WB (1:1000) #3671 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) MLC pS19 IHC (1:400) 600-401-416 rabbit pAb Thermo Fisher Scientific Inc. (Waltham, MA) MLC WB (1:1000) #8505 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (24E10) WB (1:2000); IF (1:100) #3195 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) E-Cadherin (ECCD-2) IF (1:200) #13-1900 rat mAb Thermo Fisher Scientific Inc. (Waltham, MA) FAK pY397 WB (1:1000) #3283 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) FAK WB (1:2000) #3285 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) N-Cadherin WB (1:2000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Histone H3 pS10 IF (1:800) #9701 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) PLK1 WB (1:2000); IHC (1:100); IF (1:50) #37-7000 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Paxillin pY118 WB (1:1000) #2541 rabbit pAb Cell Signaling Technology, Inc. (Danvers, MA) Paxillin WB (1:2000); IF (1:200) 610051 mouse mAb BD Transduction LaboratoriesTM (Franklin Lakes, NJ) Snail WB (1:1000) #3879 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Slug WB (1:1000) #9585 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) Vimentin WB (1:1000) #5741 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZEB1 WB (1:1000) #3396 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 WB (1:1000) #8139 rabbit mAb Cell Signaling Technology, Inc. (Danvers, MA) ZO-1 IF (1:100) #33-9100 mouse mAb Thermo Fisher Scientific Inc. (Waltham, MA) Abbreviations: WB, wWestern blot; IF, immunofluorescence; IHC, immunohistochemistry; mAb: monoclonal antibody; pAb, polyclonal antibody ..



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